Product List
Alphazyme is focused on the rapid development and customization of enzymes consumed by commercial “nucleic acid literacy” platforms. The following enzymes are available for evaluation testing, customization, or scale-up to meet specific requirements.
All Alphazyme enzymes are:
• Ready for customization (formulation, process tuning, QC specifications, fill sizes)
• Designed for scale-up
• Fully traceable within our ISO 13485:2016 compliant electronic Quality System
• BSE/TSE-free
• Available in REACH compliant formulations
• Reaction buffers can be produced and supplied if required
Codex® enzyme products, developed by Codexis and manufactured by Alphazyme, offer specific performance improvements across various genomic applications.
Alphazyme continually develops new enzymes and we welcome the opportunity to produce an enzyme specifically for your needs.
Please contact us at [email protected] to discuss your specific needs, or to request samples.
To place an order, or for more information about Alphazyme enzymes, please click here.
T4 DNA Ligase
DNA Ligase, industry standard for T/A adapter ligation, general cloning applications
T3 DNA Ligase
DNA Ligase, tolerates high salt
T4 RNA Ligase II
Double-stranded RNA Ligase
Thermolabile UDG
DNA Glycosylase from Gadus Morhua, inactivated at >55°C
Exonuclease III
Double-stranded exonuclease
DNase I (Bovine)
Digests single- and double-stranded DNA, leaving a 5'-terminal phosphate
TdT (Murine)
Non-template directed DNA polymerase; N-terminal truncation of the “S” Isoform of Murine TdT
Codex® HiFi DNA Polymerase
High fidelity (52X relative to wild-type Taq polymerase) with high uniformity of coverage
Codex® HiFi Hot-Start DNA Polymerase
High fidelity (52X relative to wild-type Taq polymerase) with aptamer-based hot start mechanism
Codex® HiFi Hot-Start 2X NGS Mix
High fidelity (52X relative to wild-type Taq polymerase), fully formulated mix for NGS library amplification with hot start enzyme, buffer, and dNTPs
Codex® HiCap RNA Polymerase
Co-transcriptional capping RNA polymerase engineered to produce synthetic mRNA at high yield and low immunogenicity
Codex® HiFi U+ DNA Polymerase
High fidelity (52X relative to wild-type Taq polymerase), uracil-literate DNA polymerase
Codex® HiTemp Reverse Transcriptase
Thermostable (up to 70°C), with high sensitivity, and reliable performance with degraded RNA
Phi29 DNA Polymerase
DNA polymerase with exceptional strand displacement, highly efficient in low-temperature (30°C) isothermal amplification systems
BPOL2 DNA Polymerase
DNA polymerase, strong strand displacement, highly effective in elevated temperature (55-65oC) isothermal amplification systems
T4 Polynucleotide Kinase
Adds (5’) or removes (3’) a phosphate at DNA termini
RNAse Inhibitor (Murine)
Broad range inhibition of RNases, including RNase A, B and C
E.coli Pyrophosphatase
Catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate
T4 GP32
Single-stranded DNA binding protein
T4 DNA Polymerase
T4 DNA Polymerase catalyzes the synthesis of a primed DNA template in the 5´→ 3´ direction. This enzyme displays a powerful 3´→ 5´ exonuclease activity, but lacks 5´→ 3´ exonuclease function.
T7 RNA Polymerase
Bacteriophage T7 RNA Polymerase is a highly processive, DNA-dependent RNA polymerase that catalyzes the in vitro transcription of a recombinant gene regulated by the T7 promoter.
Taq552
Taq552 DNA Polymerase is a truncated version of Taq DNA polymerase which lacks the N-terminal 280 amino acids of the holoenzyme. The removal of these amino acids abolishes Taq’s 5’-3’ exonuclease activity, rendering it exonuclease-free.
Exonuclease I
Exonuclease I (Exo l), is a is a highly processive single-stranded DNA-specific exonuclease which removes mono- and dinucleotides from the 3' end of single-stranded DNA molecules, leaving double-stranded molecules and the 5' end of single stranded molecules intact. Exo I requires magnesium and it is inhibited by the presence of 3'-terminal phosphate on the DNA strand.
RNAse R
E.coli RNase R is a 3´-5´ exoribonuclease that efficiently digests linear RNA molecules, leaving lariat, circular and double-stranded RNAs with short (<7 nt) 3´ overhangs intact.
Gyrase
Gyrase is a type II DNA topoisomerase that is involved in bacterial DNA supercoiling. Gyrase is the only type II isomerase that can introduce negative supercoils into DNA. It is a tetramer composed of two A and two B subunits that form a 370 kDa holoenzyme.