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Product List

Alphazyme is focused on the rapid development and customization of enzymes consumed by commercial “nucleic acid literacy” platforms. The following enzymes are available for evaluation testing, customization, or scale-up to meet specific requirements.

All Alphazyme enzymes are:
• Ready for customization (formulation, process tuning, QC specifications, fill sizes)
• Designed for scale-up
• Fully traceable within our ISO 13485:2016 compliant electronic Quality System
• BSE/TSE-free
• Available in REACH compliant formulations
• Reaction buffers can be produced and supplied if required

Codex® enzyme products, developed by Codexis and manufactured by Alphazyme, offer specific performance improvements across various genomic applications.

Alphazyme continually develops new enzymes and we welcome the opportunity to produce an enzyme specifically for your needs.

Please contact us at [email protected] to discuss your specific needs, or to request samples.

To place an order, or for more information about Alphazyme enzymes, please click here.

T4 DNA Ligase

DNA Ligase, industry standard for T/A adapter ligation, general cloning applications

T3 DNA Ligase

DNA Ligase, tolerates high salt

T4 RNA Ligase II

Double-stranded RNA Ligase

Thermolabile UDG

DNA Glycosylase from Gadus Morhua, inactivated at >55°C

Exonuclease III

Double-stranded exonuclease

DNase I (Bovine)

Digests single- and double-stranded DNA, leaving a 5'-terminal phosphate

TdT (Murine)

Non-template directed DNA polymerase; N-terminal truncation of the “S” Isoform of Murine TdT

Codex® HiFi DNA Polymerase

High fidelity (52X relative to wild-type Taq polymerase) with high uniformity of coverage

Codex® HiFi Hot-Start DNA Polymerase

High fidelity (52X relative to wild-type Taq polymerase) with aptamer-based hot start mechanism

Codex® HiFi Hot-Start 2X NGS Mix

High fidelity (52X relative to wild-type Taq polymerase), fully formulated mix for NGS library amplification with hot start enzyme, buffer, and dNTPs

Codex® HiCap RNA Polymerase

Co-transcriptional capping RNA polymerase engineered to produce synthetic mRNA at high yield and low immunogenicity

Codex® HiFi U+ DNA Polymerase

High fidelity (52X relative to wild-type Taq polymerase), uracil-literate DNA polymerase

Codex® HiTemp Reverse Transcriptase

Thermostable (up to 70°C), with high sensitivity, and reliable performance with degraded RNA

Phi29 DNA Polymerase

DNA polymerase with exceptional strand displacement, highly efficient in low-temperature (30°C) isothermal amplification systems

BPOL2 DNA Polymerase

DNA polymerase, strong strand displacement, highly effective in elevated temperature (55-65oC) isothermal amplification systems

T4 Polynucleotide Kinase

Adds (5’) or removes (3’) a phosphate at DNA termini

RNAse Inhibitor (Murine)

Broad range inhibition of RNases, including RNase A, B and C

E.coli Pyrophosphatase

Catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate

T4 GP32

Single-stranded DNA binding protein

T4 DNA Polymerase

T4 DNA Polymerase catalyzes the synthesis of a primed DNA template in the 5´→ 3´ direction. This enzyme displays a powerful 3´→ 5´ exonuclease activity, but lacks 5´→ 3´ exonuclease function.

T7 RNA Polymerase

Bacteriophage T7 RNA Polymerase is a highly processive, DNA-dependent RNA polymerase that catalyzes the in vitro transcription of a recombinant gene regulated by the T7 promoter.


Taq552 DNA Polymerase is a truncated version of Taq DNA polymerase which lacks the N-terminal 280 amino acids of the holoenzyme. The removal of these amino acids abolishes Taq’s 5’-3’ exonuclease activity, rendering it exonuclease-free.

Exonuclease I

Exonuclease I (Exo l), is a is a highly processive single-stranded DNA-specific exonuclease which removes mono- and dinucleotides from the 3' end of single-stranded DNA molecules, leaving double-stranded molecules and the 5' end of single stranded molecules intact. Exo I requires magnesium and it is inhibited by the presence of 3'-terminal phosphate on the DNA strand.


E.coli RNase R is a 3´-5´ exoribonuclease that efficiently digests linear RNA molecules, leaving lariat, circular and double-stranded RNAs with short (<7 nt) 3´ overhangs intact.


Gyrase is a type II DNA topoisomerase that is involved in bacterial DNA supercoiling. Gyrase is the only type II isomerase that can introduce negative supercoils into DNA. It is a tetramer composed of two A and two B subunits that form a 370 kDa holoenzyme.

Need a custom enzyme developed?
Need an enzyme manufactured to scale?
No problem!
Please contact us to discuss your custom project.