Product List
Alphazyme is focused on the rapid development and customization of enzymes consumed by commercial “nucleic acid literacy” platforms. The following enzymes are available for evaluation testing, customization, or scale-up to meet specific requirements.
All Alphazyme enzymes are:
• Ready for customization (formulation, process tuning, QC specifications, fill sizes)
• Designed for scale-up
• Fully traceable within our ISO 13485:2016 compliant electronic Quality System
• BSE/TSE-free
• Available in REACH compliant formulations
• Reaction buffers can be produced and supplied if required
Codex® enzyme products, developed by Codexis and manufactured by Alphazyme, offer specific performance improvements across various genomic applications.
Alphazyme continually develops new enzymes and we welcome the opportunity to produce an enzyme specifically for your needs.
Please contact us at [email protected] to discuss your specific needs, or to request samples.
T4 DNA Ligase
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5' phosphate and 3' hydroxyl groups of duplex DNA or RNA. T4 DNA Ligase joins blunt and cohesive ends, and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
Part Number
E003-B002A
Price
$1,200.00
Market Comparable Unit Concentration (U/mL)
2,000,000
Concentration (mg/mL)
2.200
Quantity (mL)
0.250
Phi29 DNA Polymerase
phi29 DNA Polymerase is a highly processive polymerase with strong strand displacement activity and a powerful 3' → 5' proofreading exonuclease activity.
Part Number
E015-B009C
Price
$1,200.00
Market Comparable Unit Concentration (U/mL)
100,000
Concentration (mg/mL)
1.000
Quantity (mL)
0.100
RNAse Inhibitor (Murine)
Murine RNAse Inhibitor binds to ribonucleases A, B, and C in a 1:1 stoichiometry with femtomolar affinity and renders them unable to bind or degrade RNA. Murine RNase Inhibitor has significantly improved resistance to oxidation compared to the human/porcine RNase inhibitors, and is stable at low DTT concentrations.
Part Number
E025-B028A
Price
$1,200.00
Market Comparable Unit Concentration (U/mL)
40,000
Concentration (mg/mL)
0.800
Quantity (mL)
1.500
T3 DNA Ligase
T3 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5' phosphate and a 3' hydroxyl termini in duplex DNA; joining blunt and cohesive end termini as well as repairing single stranded nicks in duplex DNA. T3 DNA Ligase displays a higher tolerance for salt than T4 DNA Ligase, as well as a preference for A/T overhangs (as opposed to C/G overhang pairs).
Part Number
E026-B002A
Price
$1,200.00
Market Comparable Unit Concentration (U/mL)
3,000,000
Concentration (mg/mL)
1.000
Quantity (mL)
1.200
Exonuclease III
Exonuclease III catalyzes the sequential excision of mononucleotides from 3' -hydroxyl termini of duplex DNA, or the RNA strand of a DNA/RNA hybrid. A limited number of nucleotides are removed during each binding event, resulting in coordinated progressive deletions within the population of DNA molecules.
Part Number
E033-B030A
Price
$1,100.00
Market Comparable Unit Concentration (U/mL)
100,000
Concentration (mg/mL)
1.150
Quantity (mL)
1.500
Thermolabile UDG
Thermolabile Uracil-DNA Glycosylase, originating from the species Gadus Morhua, removes uracil from DNA by hydrolyzing the N-glycosylic bond between the deoxyribose and the base leaving an AP (apurinic or apyrimidinic) site. The enzyme is useful in the prevention of amplicon carry-over and is completely and irreversibly inactivated by a 10 minute incubation at 55°C.
Part Number
E038-B002D
Price
$900.00
Market Comparable Unit Concentration (U/mL)
1,000
Concentration (mg/mL)
0.002
Quantity (mL)
3.00
T4 GP32
T4 Gene 32 Protein (T4GP32) is a single-stranded DNA (ssDNA) binding protein required for bacteriophage T4 replication and repair. It cooperatively binds to and stabilizes transiently formed regions of ssDNA and plays an important structural role during T4 phage replication.
Part Number
E045-B025A
Price
$1,200.00
Market Comparable Unit Concentration (U/mL)
--
Concentration (mg/mL)
10.000
Quantity (mL)
0.200
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of Pi from the gamma position of ATP to the 5' -hydroxyl terminus of (ds and ss) DNA, RNA and nucleoside 3' -monophosphates. PNK also acts as a 3' -phosphatase and 2', 3' cyclicphosphodiesterase.
Part Number
E047-B002D
Price
$1,200.00
Market Comparable Unit Concentration (U/mL)
10,000
Concentration (mg/mL)
0.075
Quantity (mL)
2.000
DNA Polymerase I
DNA Polymerase I (E. coli) is a template-dependent DNA polymerase with intrinsic proofreading exonuclease (3' → 5') and nick translation exonuclease (5' → 3') activities.
Part Number
E050-B037A
Price
$1,050.00
Market Comparable Unit Concentration (U/mL)
10,000
Concentration (mg/mL)
1.460
Quantity (mL)
1.200
E.coli Inorganic Pyrophosphatase
E.coli Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate (PPi) to form orthophosphate. The enzyme requires a divalent metal cation, preferably Magnesium.
Part Number
E051-B016A
Price
$800.00
Market Comparable Unit Concentration (U/mL)
100
Concentration (mg/mL)
0.030
Quantity (mL)
1.500
T4 RNA Ligase II
Double-stranded RNA Ligase
DNase I (Bovine)
Digests single- and double-stranded DNA, leaving a 5' -terminal phosphate
TdT (Murine)
Non-template directed DNA polymerase; N-terminal truncation of the “S” Isoform of Murine TdT
Codex® HiFi DNA Polymerase
Ultra-high fidelity (>600X higher than Taq Polymerase), thermostable DNA polymerase
Codex® HiFi Hot-Start DNA Polymerase
Ultra-high Fidelity, (>600X higher than Taq Polymerase), hot-start, thermostable DNA polymerase
Codex® HiFi Hot-Start 2X NGS Mix
Ultra-high fidelity, hot-start, PCR ready-mix For NGS library amplification
Codex® HiCap RNA Polymerase
Evolved RNA polymerase, adds Cap0 structure during mRNA synthesis
Codex HiFi U+ DNA Polymerase
Ultra-high Fidelity, Thermostable DNA Polymerase, Uracil-Literate
Codex® RT2
Evolved reverse transcriptase, thermostable (up to 65°C), no RNAse H activity
BPOL2 DNA Polymerase
DNA polymerase, strong strand displacement, highly effective in elevated temperature (55-65°C) isothermal amplification systems