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Associate Scientist, Protein Purification

Location: Jupiter, FL
Reports To: CSO
Status: Exempt

Position Summary
Reporting to the CSO, the Protein Purification Scientist is responsible for the design, execution, and documentation of the Company’s enzyme purification activities, including new method development/optimization and transfer to manufacturing. The candidate must have extensive experience in protein purification and analysis techniques, and a high attention to details. Responsibilities include the development and documentation of scalable, efficient enzyme purification methods, producing batches to fulfill customer project requirements, and maintaining the protein laboratory environment in a clean, orderly state in compliance with the FDA’s 21 CFR 211/820 requirements. As a senior member of the R&D team, the Senior Scientist will be responsible to manage a minimum of one Associate Scientist, whose job it is to support the Company’s protein purification projects. Alphazyme maintains a commitment to Quality and a culture of continuous improvement, and the Senior Scientist shall periodically recommend efficiency improvements to the Company’s protein production processes to management.
Job performance will involve a variety of activities including:
  • Design, implement, and optimize purification strategies to meet product design specifications
  • Preparation of all materials necessary to purify enzyme lots
  • Purify enzymes from bacterial and eukaryotic cell cultures, including cell rupture, lysate clarification, filtration/centrifugation, and column chromatography (GE AKTA systems)
  • Perform in-process contaminant profiling and activity testing, including western blot, gel electrophoresis, and protein determination
  • Capture all experimental designs, data, and observations in electronic lab notebooks
  • Complete records according to Quality System requirements
  • Ensure a clean, organized protein lab environment
  • Pack and evaluate chromatography columns
  • Communicate results at team meetings, contribute to project and experiment planning
  • Develop and maintain protocols, operational processes, and safety best practices
  • Interface and productively collaborate with members of cross-organizational teams
  • Contribute to development projects including planning, strategic direction, experimental design, data analysis and interpretation
  • Research new equipment/technologies that contribute to platform improvement
  • Troubleshoot technical issues, recommend and implement solutions
  • Minimum 5 years operational and method design experience
  • Extensive knowledge and hands-on experience in bioprocess development, protein purification and analysis
  • Experience with technology transfer to large scale production
  • Ability to complete all aspects of product batch production in a timely and accurate manner
  • Excellent organizational and communication skills
  • Team player, results oriented and able to work in multidisciplinary teams
  • Creativity, strong problem solving and critical thinking skills
  • Computer skills (Proficiency with MS Word, PowerPoint, Excel, and Access is expected)
  • Experience in a Good Manufacturing Practice (GMP) or ISO 13485 environment is desired
  • Ability to organize tasks and work independently; ability to communicate effectively orally and in writing; ability to use science literature and reference texts; ability to keep accurate records; ability to work safely
  • Minimum Bachelor’s degree with 5 years of industrial experience
About Alphazyme
Alphazyme was founded in 2018 by experts in the field of enzyme development and production with a track record of success and a passion for supporting innovators in molecular science. Our mission is to be the world’s premier partner for custom, industrial-scale molecular biology enzymes. We partner with the manufacturers of nucleic acid therapies and detection platforms to develop and produce affordable, reliable enzymes which meet the specifications of the rapidly expanding market for custom DNA and RNA molecules, genomic medicines and genetic tests. Consistent quality, scalable production, and compatibility with regulatory requirements are the hallmarks of our business.

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